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STEMCELL Technologies Inc stemspan sfem with stemspan erythroid expansion supplement
Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using <t>StemSpan</t> Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.
Stemspan Sfem With Stemspan Erythroid Expansion Supplement, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/stemspan+sfem+ii+media+with+stemspan+erythroid+expansion+supplement/erythroid+differentiation+media+stemspan+sfem+ii+supplemented+with+1%C3%97+stemspan+erythroid+expansion+supplement/pmc07142018-135-13-24
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Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using StemSpan Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.

Journal: Oncogene

Article Title: ZBTB7A prevents RUNX1-RUNX1T1-dependent clonal expansion of human hematopoietic stem and progenitor cells

doi: 10.1038/s41388-020-1209-4

Figure Lengend Snippet: Flow cytometry measurements of human bone marrow CD34+ cells stably expressing ZBTB7A WT or mutants are shown. a Representative results of cells primed for differentiation into erythrocytes using StemSpan Erythroid Expansion Supplement for 7 days. b Summary of three independent experiments. c Representative results of cells primed for differentiation into monocytes and granulocytes using HemaTox Myeloid Kit for 7 days. d Summary of three independent experiments. e Schematic representation of the effects of ZBTB7A and RUNX1–RUNX1T1 expression on hematopoietic linage commitment.

Article Snippet: A total of 5000 Human CD34+ bone marrow cells were seeded either in StemSpan SFEM with StemSpan Erythroid Expansion Supplement or HemaTox Myeloid Kit (StemCell Technologies) in a 96-well plate.

Techniques: Flow Cytometry, Stable Transfection, Expressing